pMK296 (DHC1-mAID Neo)

LOCUS       pMK296 (DHC1-mAID Neo)                9177 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  DHC1 tagging with mAID.
ACCESSION   .
VERSION     .
KEYWORDS    pMK296 (DHC1-mAID Neo)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano 
            R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, 
            Hayashi KI, Kanemaki MT.
  TITLE     The auxin-inducible degron 2 technology provides sharp degradation 
            control in yeast, mammalian cells, and mice.
  JOURNAL   Nat Commun (2020) 11(1):5701
REFERENCE   2  (bases 1 to 9177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9177
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1314..1517
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             1524..2243
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(1569..1590)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2177..2198
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2361..2482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2398..2417)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2452..2471
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2489..2944)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2971..3075
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3077..3434
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(3100..3120)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3285..3420
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3347..3366
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3469..4263
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4451..4470)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     primer_bind     complement(5610..5629)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5611..5629)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5636..5653)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5636..5652)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5645..5667)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5793..6221
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             6565..7359
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7377..8237
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7595..7614)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8408..8996
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8897..8916
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9150..9167
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccttcag cgagaggatc aaacagctgc agaacatctc actggcagct gcatctggtg
      301 gcgccaagga gctaaaggtg aaggcgctcc tgacgagtct cgggtggtca gcagctgtcc
      361 tgggctgggg tgggagtggc tctggggaaa aacacagggc ccaggtctga cctgagctcc
      421 ttcccctggg ggctgctgct ttccacagaa catccacgtg tgcctgggtg gcctgttcgt
      481 gcctgaggcg tacatcactg ccaccaggca gtatgtggcc caggccaaca gctggtccct
      541 ggaggagctc tgcctggaag tcaacgtcac cacctcacag ggcgccaccc ttgacgcttg
      601 cagcttcgga gtcacgggtg agtggagtct cacagaaaat actggctctt tgcaggtgac
      661 ctcggtggcc tgagaccatt gttcccagat acatgcactt agggtgaccg gctggcagtt
      721 gggtggagcc tctgggcgcc ctgtgactgg ggtttgtgta gctgttgtgt tcacctcagc
      781 ctgggttttg gcttccgcct cacaggtttg aaacttcaag gggccacgtg caacaacaac
      841 aagctgtcac tgtccaatgc catctcaacc gcccttcccc tgacgcagct gcgctgggtc
      901 aagcagacaa acaccgagaa gaaggccagt gtggtaagga ggcactgcct ttcccaggca
      961 ttctgcaggg acccctgcgg taacaagggc agaggcggct cctcttctat gcctgggttc
     1021 cacttggaac gggaaatgag gttcacagag gaaatccatt tcgcacctgt ccaaacctct
     1081 ccttgccggg ccccatcagc tgtcccgggc agtcttccag ttttcttact tttcccttaa
     1141 gccaccagta aacccctctg cttctgcagg taaccttacc tgtctacctg aacttcaccc
     1201 gtgcagacct catcttcacc gtggacttcg aaattgctac aaaggaggac cctcgctcgt
     1261 tctacgaaag aggtgtcgca gtcttgtgca cagagggatc cggtgcaggc gccaaggaga
     1321 agagtgcttg tcctaaagat ccagccaaac ctccggccaa ggcacaagtt gtgggatggc
     1381 caccggtgag atcataccgg aagaacgtga tggtttcctg ccaaaaatca agcggtggcc
     1441 cggaggcggc ggcgttcgtg aaggtatcaa tggacggagc accgtacttg aggaaaatcg
     1501 atttgaggat gtataaagct agcatggtga gcaagggcga ggagctgttc accggggtgg
     1561 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtccgcggcg
     1621 agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca
     1681 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcgtg gcctgcttca
     1741 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1801 acgtccagga gcgcaccatc tctttcaagg acgacggtac ctacaagacc cgcgccgagg
     1861 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1921 aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac aacgtctata
     1981 tcacggccga caagcagaag aacggcatca aggctaactt caagatccgc cacaacgttg
     2041 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2101 ccgtgctgct gcccgacaac cactacctga gccatcagtc caagctgagc aaagacccca
     2161 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg attacacatg
     2221 gcatggacga gctgtacaag taatctagat aactgatcat aatcagccat accacatttg
     2281 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     2341 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     2401 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     2461 ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc
     2521 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     2581 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     2641 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     2701 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     2761 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     2821 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     2881 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     2941 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3001 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3061 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     3121 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3181 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3241 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     3301 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3361 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3421 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     3481 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     3541 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     3601 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     3661 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     3721 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     3781 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     3841 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     3901 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     3961 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     4021 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     4081 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     4141 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     4201 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     4261 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     4321 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     4381 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     4441 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     4501 taaaaagaca gaataaaacg cacggtgttg ggtcgttgga tccacttttc tagctgcccc
     4561 tttctgtaat agtgaaagtt ggtatttaac atttattcat ttttaaaata tttggaaggt
     4621 ctgagcttgt gaaaagaaag tggttggtct gaggttggag gaagctgaat ggaatctgac
     4681 ggttgggagt ggtggaaatt ggaaggatac caggaggtat ttgggaaggc caatggcgtg
     4741 gctcctttga ggaaataaaa cactaagcat gagccggctc cgcctcttct gtctccgctt
     4801 tcatcccagg gcacagagcc ttgccttcca tgctgcccag ggagggcagc ccacggcagc
     4861 catgcccctc cccacctcgc tttcatcatg agctcgctcc cgagcggcca cagcactcat
     4921 gaatgaagac cttggggccc ttcacagaca cagatgcagc cagctgtggc tctgaaggcc
     4981 ctggggcccg ggcaccatgg ttcacacctt taatcgcagc actttgggag tctgggagtt
     5041 aaagaccagc ctcggcaaca tagtgagacc ccgtctctac aggaaattaa atcaggtgtg
     5101 gtggtgcatg cctgtagtcc cagctacttg agggctgagg tgggaggatc acccaagccc
     5161 aagaggtcga ggctgcagtg agctgtgatc tcaccactgc actccagcct gggtgacaga
     5221 gcaagaccct gtctcaaaaa aaaaaagctg ggcgtggtgg ctcatgcctg taatcccagc
     5281 actttgggag gccgaggcgg gcggatcacc aaattagccg ggcatggtgg cacatgcctg
     5341 taatcccagc tactcgggag gctgaggcag gagaattgct tgaacctggg aggcggaggt
     5401 tgcagtgagc tgaaaaaaaa aaaggcagcc cccagccgct tgtgttcttg accagggccc
     5461 caggacttgg ctcctccaga caagggagtt ttgtgctgta gatgagggag ttgcccatcg
     5521 ccgccctagc aagtccattc ccacacgacc tttccagtgg tgatgatgac agtggcccac
     5581 tcgagcatgc atctagaggg cccaattcgc cctatagtga gtcgtattac aattcactgg
     5641 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     5701 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     5761 cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc
     5821 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5881 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5941 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6001 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6061 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6121 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6181 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa ttcagggcgc
     6241 aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc
     6301 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
     6361 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
     6421 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
     6481 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa
     6541 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     6601 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     6661 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     6721 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     6781 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     6841 ctattgggcg aagtgccggg gcaggatctc ctgtcatccc accttgctcc tgccgagaaa
     6901 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6961 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     7021 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     7081 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     7141 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     7201 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     7261 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     7321 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttgaaaaagg aagagtatga
     7381 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7441 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7501 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7561 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7621 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7681 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7741 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7801 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7861 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7921 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7981 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8041 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8101 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8161 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8221 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8281 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8341 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8401 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8461 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8521 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8581 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8641 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8701 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8761 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8821 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8881 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8941 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9001 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9061 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9121 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag
//